Laurel wilt (Raffaelea lauricola) and its vector (Xyleborus glabratus): addition to the EPPO Alert List
Why: in the Southeastern part of the USA, widespread mortality of redbay (Persea borbonia) has been observed since 2003. The disease has been called ‘laurel wilt’. The cause of this tree mortality has been identified as Raffaelea lauricola, a fungus which serves as a food source for the redbay ambrosia beetle, Xyleborus glabratus (Coleoptera: Scolytidae). Both the pathogen and the insect vector originate from Asia and have shown invasive behaviour in the USA. In addition to P. borbonia, R. lauricola has been detected in avocado trees (P. americana) in Florida also causing a vascular wilt disease. As in the USA, both R. lauricola and its insect vector are perceived as a serious threat to the avocado fruit production, the EPPO Secretariat decided to add them to the EPPO Alert List.
Where: X. glabratus is native to Asia (India, Myanmar, Japan and Taiwan) where it infests aromatic tree species (mainly from the Lauraceae family). Studies of an insect collection in Beijing have shown that X. glabratus also occurs in China. R. lauricola has been isolated from X. glabratus beetles collected from Kyushu Island (Japan) and Taiwan. However, differences in the mycangial mycoflora of X. glabratus in Taiwan, Japan and USA, suggest that the X. glabratus population established in the USA probably originates from another part of Asia. In the USA, X. glabratus was first caught in May 2002 in a trap located at Port Wentworth (near Savannah, Georgia). Soon after, widespread mortality of redbay (P. borbonia) was observed in the coastal plains of Georgia and other US states. In 2007, R. lauricola was detected for the first time in an avocado tree in Jacksonville, Florida. As of July 2013, R. lauricola has been detected in 90 avocado trees in various commercial groves of Florida, and more than 1 900 symptomatic trees have been removed as part of a suppression and sanitation strategy. Interestingly, only 6 X. glabratus beetles could be caught in these avocado groves.
EPPO region: absent.
Asia: Japan, Taiwan.
North America: USA (Alabama, Florida, Georgia, Mississippi, North Carolina, South Carolina).
EPPO region: absent.
Asia: Bangladesh, China (Fujian, Hunan, Sichuan), India (Assam, West Bengal), Japan (Kyushu), Myanmar, Taiwan.
North America: USA (Alabama, Florida, Georgia, Mississippi, North Carolina, South Carolina).
On which plants: laurel wilt has been observed in Lauraceae (in Asia, the fungus has been detected only in the vector for the moment). R. lauricola infects P. borbonia (redbay), P. palustris (swampbay), Sassafras albidum and P. americana (avocado). In USA, P. borbonia and P. palustris are common, shade tolerant, broadleaved evergreen trees of various forested habitats and residential areas of the Southeastern Atlantic coastal plain region. R. lauricola has also been isolated from Lindera melissifolia, Litsea aestivalis and Lacaria trianda which are considered as endangered species but the impact of laurel wilt on these trees remains uncertain.
In its native range, X. glabratus is often associated with Lauraceae (e.g. Cinnamomum camphora, C. osmophloeum, Lindera latifolia, Litsea elongata, Machilus nanmu, Phoebe lanceolata, Phoebe neurantha, Phoebe zhennan). However, it is also found on trees belonging to other plant families such as: Leucaena glauca and Lithocarpus edulis (Fagaceae), Schima superba (Theaceae), Shorea robusta (Dipterocarpaceae). In the USA, X. glabratus has been found attacking P. americana (avocado), P. borbonia (redbay), P. palustris (swampbay) and Sassafras albidum.
Damage: symptoms of laurel wilt are typical of those that are caused by other vascular wilt pathogens: vascular black discoloration, rapid wilting, necrosis of foliage and defoliation. It has been shown that xylem function and hydraulic conductivity were significantly impaired by R. lauricola infection. Glasshouse and field experiments conducted on several avocado cultivars have also shown that a single inoculation resulted in most cases, in extensive colonization and symptom expression. It is not known how many individuals of X. glabratus are needed to result in lethal infection, but observations suggest that one or only a few beetles are required. Laurel wilt has caused widespread mortality of P. borbonia and P. palustris in the USA, killing nearly all trees within 3-5 years after X. glabratus becomes established and detected. In some areas, up to 90% tree mortality has been noticed. In some areas in Georgia, the composition of forest communities was altered by R. lauricola, as after the destruction of P. borbonia, other tree species (e.g. Magnolia virginiana and Gordonia lasianthus) became dominant.
X. glabratus makes pinhole-sized entrance holes in the bark that either bleed or produce light-coloured boring dust. It bores characteristic galleries in the wood of infested trees. X. glabratus can produce frass tubes that resemble ‘tooth picks’ extending out from the bark. However, it is more common to see piles of sawdust around the base of the tree than the tubes themselves. Adults are small beetles, 2-3 mm long, slender, and brown-black in colour. Larvae are white coloured, c-shaped, legless with an amber-coloured head capsule. Little information is available about the life cycle of X. glabratus. Generation time is uncertain but observations indicated that brood development can occur in 50-60 days in Southeastern USA. Ambrosia beetles are usually found on dead or weakened trees, but X. glabratus is able to attack healthy trees. It is considered that young trees with a diameter less than 2.5 cm cannot sustain populations of X. glabratus.
Dissemination: R. lauricola moves primarily via its vector, X. glabratus. Fungal spores are carried in the insect mycangia (specialized structures found at the base of each mandible) and are dispersed in the xylem as the adult female constructs her galleries and lay eggs. Adults and larvae feed on the conidia produced by the fungus. In the USA, it is suspected that X. glabratus (carrying R. lauricola) was introduced with wood packing material from Asia. Once introduced, the movement of infested firewood is considered to be an important means of dissemination within the USA. The survival of X. glabratus and R. lauricola in wood chips made from infested P. borbonia trees has been studied. Chipping can significant reduce the number of X. glabratus and limit the persistence of R. lauricola but does not completely eliminate them.
Pathway: Plants for planting, wood and bark, wood chips, wood packaging material of host trees from countries where R. lauricola and X. glabratus occur. According to preliminary studies, avocado fruit is not a pathway.
Possible risks: Avocado is not widely grown in the EPPO region but is of economic importance at least in Israel and Spain. Laurel forests (including Lauraceae genera such as Apollonias, Ocotea, Persea) are found in the Azores, Madeira (PT) and Islas Canarias (ES). Although their susceptibility to R. lauricola and X. glabratus is not known, they are of high patrimonial value. The insect vector, X. glabratus has cryptic habits and is therefore difficult to detect. In the USA, laurel wilt is perceived as a serious threat to the avocado production. In Florida, expected losses in the absence of control measures were estimated at 27 to 54 million USD. In infected avocado groves, control strategies rely on the following methods: destruction of infected trees, control of X. glabratus (insecticides, attractants (e.g. manuka and phoebe oil lures) or repellents), and severing of root grafts. For the moment, it seems that no tolerant or resistant avocado cultivars are available.
Finally, recent studies have shown that the transfer of R. lauricola from diseased trees to insects other than X. glabratus was possible (e.g. Xyleborus affinis, Xyleborus ferrugineus, Xyleborus volvulus, Xyleborinus gracilis, Xyleborinus saxeseni, Xylosandrus crassiusculus), and that transmission to healthy trees was in some cases obtained. Although more studies are needed to confirm these preliminary results, this indicates that ‘new’ vectors might play a role in the disease epidemiology. Considering the significant mortality observed in the USA on several Lauraceae species and the general lack of effective control measures, it is desirable to avoid the introduction of R. lauricola and its vector, X. glabratus in the EPPO region.
EPPO RS 2014/086
Panel review date -/- Entry date 2014-05
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