Multiplex RT-PCR to detect eight stone fruit viruses
A one step RT-PCR assay was developed to detect and differentiate the following 8 viruses of stone fruit trees: Apple mosaic ilarvirus (EU Annexes), Prunus necrotic ringspot ilarvirus, Prune dwarf ilarvirus, American plum line pattern ilarvirus (EPPO A1 list), Plum pox potyvirus (EPPO A2 list), Apple chlorotic leaf spot trichovirus, Apricot latent foveavirus, Plum bark necrosis stem pitting associated ampelovirus. This multiplex RT-PCR was considered an efficient, sensitive and rapid tool, which can allow routine detection of a large number of viruses either alone or in combination.
Sánchez-Navarro JA, Aparicio F, Herranz MC, Minafra A, Myrta A, Pallás V (2005) Simultaneous detection and identification of eight stone fruit viruses by one-step RT-PCR. European Journal of Plant Pathology 111(1), 39-46.