EPPO Global Database

EPPO Reporting Service no. 02 - 2006 Num. article: 2006/039

International course on ‘The use of real-time BIO-PCR for detecting brown rot and ring rot bacteria on potatoes’, Ankara, 2006-06-26/30

An international course on the use of RealTime BIO-PCR to detect brown rot (Ralstonia solanacearum) and ring rot (Clavibacter michiganensis subsp. sepedonicus) will be organized by the Plant Protection Central Research Institute of Ankara in collaboration with the Foreign Diseases and Weed Science Research Unit, USDA Fort Detrick, USA. The course will be hosted at the Campus of Agriculture in Ankara and will be held over a period of 5 days from the  26th to 30th of June, 2006, in morning and afternoon sessions. Lecturers will be Dr. Norman W. Schaad (USDA, USA) and Dr. Meriç Özakman (PPCRI, Turkey). This course is addressed to professionals who are already directly involved in the detection, diagnosis and research on plant pathogenic bacteria. The official language of the course will be English and no translation will be made. The deadline for the submission of application is 12 May 2006. Registration fees of the course amount to 300 euros. This sum covers tuition fee, leaflets and lunches during the course. Hotel reservation will be made for the participants. The course is limited to 10 participants.

Theoretical part
  • Introduction
  • Recent information on Ralstonia solanacearum
  • Recent information on Clavibacter michiganensis subsp. sepedonicus
  • Strategies for detecting bacteria
  • Role of detection in quarantine programs
  • Real-time PCR
  • Real-time BIO-PCR
Practical part
  • Extraction of bacteria from potato tubers
  • Preparing PCR mixtures and agar media for BIO-PCR
  • Programming and using Cepheid SmartCycler
  • RealTime PCR
  • Evaluation of results


Meriç Özakman

Plant Protection Central Research Institute
PO Box: 49 Yenimahalle 06172 Ankara, TURKEY
Tel: +90-312-344 5994 ext:159
Fax: +90-312-315 1531
E-mail: meric_ozakman@zmmae.gov.tr


Personal communication with Dr Ozakman (PPCRI, Ankara), 2006-02.