Potexvirus citriflavimaculae, a new Mandarivirus associated with vein yellowing and mottling of citrus
Potexvirus citriflavimaculae (citrus yellow mottle-associated virus, CiYMaV) was first identified by high throughput sequencing from 74 Citrus spp. trees in Punjab Province, Pakistan in 2018, and later by molecular testing in 18 samples of Citrus reticulata from 3 orchards in New Delhi, India in November 2022. Infected trees showed moderate to severe vein yellowing and leaf mottling. Koch’s postulates were confirmed on C. maxima and C. reticulata x C. sinensis. In Pakistan, infections were observed in the absence of other known citrus viruses and CiYMaV was confirmed to infect C. reticulata, C. x limon, C. sinensis and C. maxima. As some Citrus spp. trees did not display any symptoms, further investigations are needed to determine which Citrus species are symptomatic. In samples collected in India all samples showed co-infection with citrus yellow vein clearing virus (Potexvirus citriflavivenae, CYVCV, EPPO Alert list), therefore Kumar et al. (2024) suggest P. citriflavimaculae may be present in other countries, such as China, where symptoms are attributed to CYVCV.
Sources
Kumar R, Gupta N, Sharma SK, Kishan G, Srivastava N, Khan ZA, Kumar A, Baranwal VK (2024) Mixed infection of two mandariviruses identified by high-throughput sequencing in kinnow mandarin and development of their specific detection using duplex RT-PCR. 3 Biotech. 14(6), 170.
Wu J, Zhang S, Atta S, Yang C, Zhou Y, Di Serio F, Zhou C, Cao M (2020) Discovery and survey of a new mandarivirus associated with leaf yellow mottle disease of citrus in Pakistan. Plant Disease 104 (6), 1593-1600. https://doi.org/10.1094/PDIS-08-19-1744-RE
Wu J, Zhang S, Liang X, Xing F, Atta S, Wang X, Cao M (2023) Development and pathogenicity analysis of full-length infectious cDNA clones of citrus yellow mottle-associated virus in citrus plants. Journal of Integrative Agriculture. 22(10), 3034-3041. https://doi.org/10.1016/j.jia.2023.08.014