Recommendations to policy makers from Euphresco projects
The following research project has recently been carried out in the framework of Euphresco (network for phytosanitary research coordination and funding – hosted by EPPO). The report presenting the main objectives and results of this project, as well as recommendations made to policy makers, can be viewed on the Internet.
Comparison of multiple real-time PCR & real-time LAMP detection methods for the plant pathogen ‘Candidatus Liberibacter' spp. causing the Huanglongbing disease on Citrus spp. (HLBVALID)
Visual inspection of symptomatic plants is a routine method for the surveillance of huanglongbing (citrus greening) disease, but symptoms can be misinterpreted. Conventional PCR tests can lead to false negative results due to the low titre and uneven distribution of the bacterium in the host plant, especially at an early stage of infection. Several real-time PCR tests have been developed recently. The project aimed to compare the performance of three real-time PCR tests (Bertolini et al., 2014; Li et al., 2006; Morgan et al., 2012) and the conventional duplex PCR from Teixeira et al. (2005) and Hocquellet et al. (1999). The real-time PCR test from Li et al. (2006) and the conventional duplex PCR performed best for both 'Candidatus Liberibacter asiaticus' and 'Candidatus Liberibacter africanus'.
Authors: Cellier G, Cruz L, Sá Pereira P, Andrade E, Cubero J, Redondo C, Sabuquillo P, Roselló M, Devorshak C, D'Onghia AM, Yaseen T, Ince E, Nilüfer Y, Güler PG.
Duration of the project: from 2016-09-01 to 2019-04-30.
Report: https://zenodo.org/record/3243371#.XP_Z5lwzbct
Sources
Euphresco (2019-06). https://www.euphresco.net/projects/