Citrus tristeza virus
Serological methods are mostly used to detect CTV in citrus in Spain, and a new method has now been tried out: polyacrylamide gel electrophoresis of ds-RNA. Distinctive and reproducible electrophoretic patterns were obtained for 24 isolates. It was hoped that mild and severe isolates could be distinguished by this method (which they are not by the serological methods), but there was no consistent correlation between severity and ds-RNA pattern. However, one recently introduced strain causing seedling yellows symptoms could be distinguished from the other 23 Spanish CTV isolates.
Sources
Moreno P. & Guerri J. Characterization of Spanish isolates of CTV by ds-RNA. Proceeding of the 7th Congress of the Mediterranean Phytopathological Union, Granada (ES), September 1987.