EPPO Global Database

EPPO Reporting Service no. 05 - 1991 Num. article: 1991/14

Differentiation of potato cyst nematodes


In the Netherlands, species-specific monoclonal antibodies have been developed to differentiate between Globodera pallida and G. rostochiensis.
Basing on these monoclonal antibodies an ELISA test has been developed which allows the detection and differentiation of potato cyst nematodes at even very low levels.
This ELISA test is now routinely used by the Dutch inspection services.


Sources

Schots, A.; Bakker, J.; Bovenkamp van de, G.W.; Stolte, T.; Luimes, J.; Haan de, E.; Minnaar, R.; Gommers, F.J.  (1991)
Identification and quantification of Globodera rostochiensis and G. pallida in soil samples using an ELISA based on specific monoclonal antibodies.
Abstracts of 'Diagnostics in Applied Biology', Nottingham March 1991, Association of Applied Biologists.