Serological detection of tomato spotted wilt virus
Direct tissue blotting, enzyme-linked immunosorbent assay (ELISA) and dot-blot immunoassay (DBIA) were compared in their ability to detect tomato spotted wilt virus.
The virus was readily detected in leaves of infected Nicotiana benthamiana by direct tissue blotting. Direct tissue blotting and DAS-ELISA tested positively stem and leaf samples of Eustoma and Impatiens showing viruslike symptoms. Asymptomatic leaves and stems from the same plants were tested negatively by ELISA and positively by direct tissue blotting.
DBIA was eight times more sensitive than ELISA to detect TSWV in extracts of infected N. benthamiana.
Sources
Hsu, H.T. & Lawson, R.H. (1991) Direct tissue blotting for detection of tomato spotted wilt virus in Impatiens.
Plant Disease 75, 292-295.