Escherichia coli cloned with BT toxin becomes toxic to Leptinotarsa decemlineata
In Germany, a DNA fragment encoding an insecticidal toxin was isolated from Bacillus thuringiensis subsp. tenebrionis. The gene was inserted into several vector plasmids and expressed in Escherichia coli whose cell extracts later became insecticidal to Leptinotarsa decemlineata (EPPO A2 pest) larvae.
Rhim, S.L.; Jahn, N.; Schnetter, W.; Geider, K. (1990) Heterologous expression of a mutated toxin gene from Bacillus thuringiensis subsp. tenebrionis.
FEMS Microbiology Letters 66, 95-99.