Detection of Xanthomonas oryzae pv. oryzae
In Japan, investigations were carried out to detect through specific antibodies extracellular polysaccharides from Xanthomonas oryzae pv. oryzae (EPPO A1 quarantine pest) by immunofluorescent and immunoelectron microscopy and ELISA.
The developed antibody detected extracellular polysaccharide of the pathogen at concentrations above 0,1 æg/ml using ELISA and did not react with extracellular polysaccharides from X. campestris pv. campestris and X. citri pv. citri. Immunofluorescent antibody staining of infected rice leaves showed that extracellular polysaccharide produced by X.o. pv. oryzae was distributed in both xylem vessels and transverse veins, but not in either sieve tubes or mesophyll tissues. The distribution of extracellular polysaccharide coincided with that of bacteria in the infected tissues. Immunoelectron microscopic observations showed that the localization of extracellular polysaccharide was restricted in the area close to bacterial cells.
Sources
Watabe, M.; Kitamura, S.; Horino, O. (1993) Immunohistochemical studies on extracellular polysaccharide from Xanthomonas campestris pv. oryzae in infected rice leaves.
Presentation at the 6th International Congress of Plant Pathology, Montreal, CA, 1993-07/08-27/06.