Detection of MLO's in Scaphoideus titanus by dot hybridization
In Italy experiments were carried out to detect mycoplasma-like organisms in Scaphoideus titanus leafhoppers which were reared on grapevine, infected by grapevine flavescence dorée MLO (EPPO A2 quarantine pest). Using dot hybridization with biotinylated MLO-DNA probes it was possible to detect MLOs in the vector of the disease. Leafhoppers reared on asymptomatic, but infected grapevines also carried MLOs. The authors assumed that since many batches of leafhoppers gave positive signals to several probes it is possible that several different MLOs are involved in the flavescence etiology.
Sources
Bertaccini, A.; Arzone, A.; Alma, A.; Vibio, M. (1993) Detection of mycoplasmalike organisms in Scaphoideus titanus reared on flavescence dorée infected grapevine by dot hybridization using DNA probes.
Phytopatologia Mediterranea 32, 20-24