Serological detection of Clavibacter michiganensis subsp. sepedonicus
The serological detection of several strains of Clavibacter michiganensis subsp. sepedonicus (EPPO A2 quarantine pest) was investigated in North Dakota, US. The potential to detect mucoid, intermediate and nonmucoid strains of the bacterial ring rot pathogen by enzyme-linked immunosorbent assay (ELISA) from infected potato stem samples was investigated using six different combinations of C. m. sepedonicus antibodies. It was found that the mucoid and intermediate strains of the disease could be easily detected by ELISA, mucoid strains, however, were not detectable by this method.
All strains were detected from infected potato stems using the technique of indirect fluorescent antibody staining.
Sources
Baer, D.; Gudmestad, N.C. (1993) Serological detection of nonmucoid strains of Clavibacter michiganensis subsp. sepedonicus in potato.
Phytopathology 83, 157-163.