Detection of Xanthomonas campestris pv. dieffenbachiae
A "miniplate system" has been developed in Hawaii which allows detection of Xanthomonas campestris pv. dieffenbachiae (potential EPPO A2 pest) in large numbers of samples. Tissue exudates are applied to individual wells of 96-well tissue-culture plates filled with 150 æl of esculin-trehalose medium, which promotes the growth of X.c. pv. dieffenbachiae and turns brown, indicative of esculin hydrolysis. The identity is then confirmed by an ELISA test using X.c. pv. dieffenbachiae-specific monoclonal antibodies. The predictive value of a positive result for the "miniplate system" was 97.9%.
Author's address:
University of Hawaii
Honolulu, HI, 96822
USA
Sources
Normann, D.; Lipp, R.; Benedict, A.; Alvarez, A. (1992) Enhanced detection of Xanthomonas campestris pv. dieffenbachiae using a "miniplate system".
Presentation at the 1992 APS/MPS Annual Meeting, Portland, OR, USA. Phytopathology 82, 1177.