New geminiviruses transmitted by Bemisia tabaci
In the USA, experiments were carried out to detect and characterize Bemisia tabaci (EPPO A2 pest)-transmitted geminiviruses through polymerase chain reaction (PCR).
PCR methods were developed that allowed the amplification of viral DNA fragments from the two components, DNA-A or DNA-B, for previously undescribed geminiviruses. The technique resulted in the detection of 10 new geminiviruses from tomato from Mexico, Costa Rica and the Dominican Republic and the detection of geminiviruses ;from Sida sp. and Calopogonium sp. from Costa Rica; from soybeans from Puerto Rico; from Macroptilium lathyroides from the Dominican Republic and from cassava and a legume weed from Malawi. For four of these viruses names were proposed: sida golden mosaic geminivirus, macroptilium golden mosaic geminivirus, calopogonium golden mosaic geminivirus and soybean golden mosaic geminivirus.
Rojas, M.R.; Gilbertson, R.L.; Russel, D.R.; Maxwell, D.P. (1992) Detection and characterization of whitefly-transmitted geminiviruses by use of polymerase chain reaction.
Presentation at the 1992 APS/MPS Annual Meeting, Portland, OR, USA. Phytopathology 82, 1087.