Detection of tomato spotted wilt tospovirus
In the USA, experiments were carried out to study the effects of storage temperature and infection age of tomato spotted wilt tospovirus (potential A2 pest) in tomato leaves.
Greenhouse-grown tomato plants were mechanically inoculated with TSWV four weeks after transplanting. After 5 - 9 weeks leaves from symptomatic plants, tested positive for TSWV, were stored at various temperatures and periodically tested by ELISA for TSWV. The virus could be detected through 21 days of storage at -17 to 5ø C and through 6 - 7 days at 23, 27 or 32ø C. The ELISA values decreased significantly after 5 days at 32øC and the infectivity was lost after one day at 32ø C.
Carey, D.K.; Mullen, J.M.; Gudauskas, R.T. (1992) Effects of storage temperature and infection age of tomato spotted wilt tospovirus L-strain in tomato leaves.
Presentation at the 1992 APS/MPS Annual Meeting, Portland, OR, USA. Phytopathology 82, 1106.