Simultaneous detection of six viruses of tomato
Studies have been carried out in Spain to develop a polyprobe for the simultaneous detection by molecular hybridization of six tomato viruses which can occur in single or multiple infections in commercial crops:
- Cucumber mosaic virus (Cucumovirus, CMV),
- Parietaria mottle virus (Ilarvirus, PMoV),
- Pepino mosaic virus (Potexvirus, PepMV – EPPO Alert List),
- Potato Y virus (Potyvirus, PVY),
- Tomato mosaic virus (Tobamovirus, ToMV),
- Tomato spotted wilt virus (Tospovirus, TSWV – EPPO A2 List).
The sensitivity and specificity of the polyprobe were found equivalent to those of individual probes. The polyprobe could also be used in combination with tissue-printing as a sample preparation technique. The authors concluded that this new method (polyprobe/tissue printing) could be a useful tool for the routine screening to tomato plants to verify the presence of these viruses (alone or in combination).
Sources
Aparicio F, Soler S, Aramburu J, Galipienso L, Nuez F, Pallás V, López C (2009) Simultaneous detection of six RNA plant viruses affecting tomato crops using a single digoxigenin-labelled polyprobe. European Journal of Plant Pathology 123(1), 117-123.