Detection of Xanthomonas campestris pv. vesicatoria by PCR in pepper and tomato seeds
A PCR method has been developped in USA to detect Xanthomonas campestris pv. vesicatoria (EPPO A2 quarantine pest) associated with pepper (Capsicum annuum) and tomato (Lycopersicon esculentum) seeds. Oligonucleotide primers specific for the hypersensitive reaction and pathogenicity (hpr) gene cluster were used in the reaction. The minimum number of cells which could be detected in washes from pepper or tomato seeds was from 102 to 103 cfu per ml (which is approximately 1,000 times fewer than the minimum number of cells detected by ELISA).
Leite, R.P.Jr; Jones, J.B.; Somodi, G.C. (1995) Detection of Xanthomonas campestris pv. vesicatoria associated with pepper and tomato seed by DNA amplification.
Plant Disease, 79(9), 917-922.