Existence of two major groups of isolates of plum pox potyvirus
The variability of 28 isolates of plum pox potyvirus (EPPO A2 quarantine pest) from several European and Mediterranean countries has been studied by using three different methods (electrophoretic properties, antigenic properties of the N- and C- parts of the coat protein, RFLP analysis of a PCR amplified cDNA fragment corresponding to the 3' end coat protein gene). It can be recalled that two serological isolates have previously been identified: PPV-M (Markus strain), a peach isolate introduced from Northern Greece which severely reduced the growth of GF 305 peach seedlings, and PPV-D (Dideron strain) an apricot isolate from southeastern France inducing less reduction of plant growth. Each of the three methods gave similar results. They were all effective and showed a close correlation in the typing of the 28 isolates. This confirms the existence of two major groups of isolates designated as PPV-D and PPV-M.
Sources
Bousalem, M.; Candresse, T.; Quiot-Douine, L.; Quiot, J.B. (1994) Comparison of three methods for assessing plum pox virus variability: further evidence for the existence of two major groups of isolates.
Journal of Phytopathology, 142 (2), 163-172.