EPPO Global Database

EPPO Reporting Service no. 01 - 1995 Num. article: 1995/20

Detection of geminiviruses from tropical countries

By using DAS-ELISA and two monoclonal antibodies prepared against particles of a Nigerian isolate of African cassava mosaic geminivirus, it was possible to differentiate African geminiviruses (tomato yellow leaf curl from Senegal (EPPO A2 quarantine pest); African cassava mosaic virus from West and East Africa, okra leaf curl from Côte d'Ivoire), from those originating in other parts of the world (Euphorbia mosaic virus from America, Indian cassava mosaic virus and tomato yellow leaf curl both from India). The authors concluded that these two monoclonal antibodies can allow the detection of whitefly-transmitted geminiviruses from various plants and various geographical origins and could be used on a large scale for epidemiological, sanitation or resistance programmes. However, when the full identification of the virus is needed other tests should be performed (symptomatology, transmission and host range).


Givord, L.; Fargette, D.; Kounouguissa, B.; Thouvenel, J.C.; Walter, B.; Van Regenmortel, M.H.V (1994) Detection of geminiviruses from tropical countries by a double monoclonal antibody ELISA using antibodies to African cassava mosaic virus.
Agronomie, 14 (5), 327-333.