Specific ELISA detection of the Andean strain of potato S carlavirus
Potato S carlavirus (PVS) affects many cultivars of potatoes worldwide. However, some variants from several South American potato cultivars have been described and designated as the Andean strain of potato virus S (PVSA). They produced systemic mottle symptoms and necrosis in Chenopodium quinoa, and are transmitted more effectively by aphids or by contact than the isolates usually occurring in European countries. In UK, isolates of PVSA have been reported in some breeder's selections and potato cultivars imported for trial (Dolby & Jones, 1987). As there is some concern about the spread of PVSA, the authors stressed the need for rapid diagnosis. An ELISA test using four monoclonal antibodies specific of PVSA has been developed in Czech Republic. These monoclonal antibodies reacted with four isolates of PVSA but not with six other viruses of the carlavirus group. The authors felt that these monoclonal antibodies could be used to produce specific diagnostic kit for routine detection of PVSA.
Cerovska, N.; Filigarova, M. (1995) Specific detection of the Andean strain of potato virus S by monoclonal antibodies.
Annals of Applied Biology, 127(1), 87-93.
Dolby, C.A.; Jones, R.A. (1987) Occurrence of the Andean strain of potato virus S in imported potato material and its effects on potato cultivars.
Plant Pathology, 36, 381-388.