Monoclonal antibodies specific to M or D serotypes of plum pox potyvirus
European isolates of plum pox potyvirus (PPV - EPPO A2 quarantine pest) fall into two main groups which can be differentiated because of molecular properties (electrophoretic mobility of dissociated coat protein, restriction site polymorphism in the coat protein gene). These PPV groups, originating from Eastern and Western Europe, respectively, proved to be related to the PPV serotypes M and D. Specific detection of the different serotypes is important as their epidemiology differs. PPV-M is more aggressive on peach, and spreads more rapidly in orchards. It is readily transmitted by aphids to peach, plum and apricot, whereas PPV-D is little or not transmissible to and between peach trees. So far, specific monoclonal antibodies were only available for PPV-D. However, recently, a monoclonal antibody to an Albanian isolate of PPV was obtained and it specifically recognizes PPV-M.
Sources
Boscia, D.; Zeramdini, H.; Cambra, M.; Potere, I.; Gorris, M.T.; Myrta, A.; Di Terlizzi, B.; Savino, V. (1997) Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus.
European Journal of Plant Pathology, 103(5), 477-480.