Detection and eradication of chrysanthemum stunt viroid in Tenerife (ES)
In a propagation glasshouse located on Tenerife island (Islas Canarias, Spain), several plants of chrysanthemum (cv. Teide) were stunted and flowered 10 days earlier than the rest of the plants within the same plot. By using electrophoresis and molecular hybridization, chrysanthemum stunt viroid (EPPO A2 quarantine pest) was detected in symptomatic plants. Eradication measures were taken. An imprint-hybridization method (with digoxigenin-labelled probes) was used to survey chrysanthemum crops on the island during the course of the eradication of the viroid. Tests were done on 39 cultivars and chrysanthemum stunt viroid was detected in 6 cultivars. The affected plants were traced to a shipment of mother plants from a single source. All affected plants were destroyed. No other outbreak of chrysanthemum stunt viroid has been reported.
Duran-Vila, N.; Romero-Durbán , J.; Hernandez, M. (1996) Detection and eradication of chrysanthemum stunt viroid in Spain.
Bulletin OEPP/EPPO Bulletin, 26(2), 399-405.