PCR detection method for Colletotrichum acutatum on strawberry
A PCR method for the detection of Colletotrichum acutatum (EU Annex II/A2) has been developed in Northern Ireland (UK). Specific primers amplified a 490 bp fragment from several isolates of C. acutatum but not from other members of the genus Colletotrichum. With these primers, amplification was also obtained from DNA extracted from strawberry tissues infected by C. acutatum. Southern hybridization analysis confirmed that the 490 bp fragments from C. acutatum DNA and from infected strawberry are identical.
Sources
Sreenivasaprasad, S.; Sharada, K.; Brown, A.E.; Mills, P.R. (1996) PCR-based detection of Colletotrichum acutatum on strawberry.
Plant Pathology, 45(4), 650-655.