New detection method for potato spindle tuber viroid
A new detection method has been developed in Germany for potato spindle tuber viroid (EPPO A2 quarantine pest). The method is based on immobilization of plant sap on filter paper, by dotting or tissue printing followed by RT-PCR. The print PCR was shown to be suitable for testing large samples of potato plants, whereas dot PCR was recommended for in vitro plantlets and tuber tissue. It was also found that the dotted or imprinted papers can be stored at 4 °C for at least two weeks (in Triton X-100 solution or under dry conditions) but that they should not be stored at room temperature.
Weidemann, H.L.; Buchta, U. (1998) A simple and rapid method for the detection of potato spindle tuber viroid (PSTVd) by RT-PCR.
Potato Research, 41(1), 1-8.