Detection method for cherry little cherry ‘closterovirus’
An RT-PCR method has been developed for the detection of cherry little cherry ‘closterovirus’ (EU Annex II/A1 for non-European isolates). Several European isolates of cherry little cherry were compared by partial sequence analysis and dsRNA gel electrophoresis and the results showed high homology between them. The authors felt that the RT-PCR method may be applicable for the detection of a wide range of isolates (at least from Europe) and could be a useful tool for quarantine and certification purposes.
Sources
Vitushkina, M.; Fechtner, B.; Agranovsky, A.; Jelkmann, W. (1997) Development of an RT-PCR for the detection of little cherry virus and characterization of some isolates occurring in Europe.
European Journal of Plant Pathology, 103(9), 803-808.