EPPO Global Database

EPPO Reporting Service no. 05 - 1999 Num. article: 1999/089

Identification methods for Bursaphelenchus species

Reliable methods for the differentiation of Bursaphelenchus xylophilus (EPPO A1 quarantine pest) from other Bursaphelenchus species associated with pine trees are essential for quarantine purposes. A molecular technique using RFLP analysis of the amplified internal transcribed spacer region of ribosomal DNA (ITS-RFLP) has been successfully developed to differentiate between: Bursaphelenchus xylophilus, B. mucronatus, B. fraudulentus, B. leoni and B. sexdentati. This method could also differentiate between the 2 intraspecific forms of B. mucronatus.


Hoyer, U.; Bergermeister, W.; Braasch, H. (1998) Identification of Bursaphelenchus species (Nematode, Aphelenchoididae) on the basis of amplified ribosomal DNA (ITS-RFLP).
Nachrichtenblatt des Deutschen Pflanzenschutzdienstes, 50(11), 273-277.