Localization of tobacco ringspot nepovirus in Xiphinema americanum sensu stricto
An indirect immunofluorescent technique has been developed in USA to localize tobacco ringspot nepovirus (EPPO A2 quarantine pest) in the vector Xiphinema americanum sensu stricto. This method is rapid and tomato ringspot nepovirus can be visualized in retention regions of the nematode. In addition to the fact that the detection of the virus is highly correlated with actual virus transmission, the authors noted that this method could provide an efficient tool to study the transmission process of viruses by X. americanum
Sources
Wang, S.; Gergerich, R.S. (1998) Immunofluorescent localization of tobacco ringspot nepovirus in the vector nematode Xiphinema americanum.
Phytopathology, 88(9), 885-889.