Colorimetric PCR to detect viruses in woody plants
A colorimetric PCR technique has been developed in California (US). It is based on the use of PCR in which gel electrophoresis or molecular hybridization to detect virus-specific products is replaced by a colorimetric reaction in ELISA plates. This technique was successfully used to detect the following viruses in woody and herbaceous plants: a walnut isolate of cherry leaf roll nepovirus, citrus tristeza closterovirus (EPPO A2 quarantine pest), prunus necrotic ringspot ilarvirus and tomato ringspot nepovirus (EPPO A2 quarantine pest). The method can also provide quantification of the viruses.
Rowhani, A.; Biardi, L.; Routh, G.; Daubert, S.D.; Golino, D.V. (1998) Development of a sensitive colorimetric-PCR assay for detection of viruses in woody plants.
Plant Disease, 82(8), 880-884.