Differentiation between Monilinia laxa, M. fructigena and M. fructicola by using total mycelial protein SDS-PAGE
An electrophoresis method to distinguish between Monilinia laxa, M. fructigena and M. fructicola (EPPO A1 quarantine pest) has been developed in Italy. 46 isolates of Monilinia laxa, M. fructigena and M. fructicola from various parts of the world (Asia, Europe, North America, Oceania) were compared using SDS-PAGE of total mycelial proteins. Results showed that total mycelial protein profiles can be used to differentiate between the 3 species of Monilinia. Only two isolates from Australia presented atypical profiles. The authors concluded that total mycelial protein SDS-PAGE analysis is a generally reliable method to discriminate between the 3 species, apart from very few ambiguous cases for which further analysis with other methods is necessary.
Sources
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Belisario, A.; Luongo, L.; Corazza, L. (1999) Identification of Monilinia species by total mycelial protein SDS-PAGE.
Phytopathologia Mediterranea, 38(3), 115-121.
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