New detection method for Clavibacter michiganensis subsp. sepedonicus
A new detection method for Clavibacter michiganensis subsp. sepedonicus (EPPO A2 quarantine pest) has been developed in USA. It is based on the use of BIO-PCR and an automated PCR detection system (TaqMan). The method was tested on 30 naturally infected ring rot suspect tubers, on other Clavibacter michiganensis subspecies, related Gram-positive bacteria and 150 unknown bacteria isolated from potato tubers. The method was found highly specific, sensitive, reliable and rapid (3 days for total assay). This new method can routinely be applied to large numbers of potato tubers.
Schaad, N.W.; Berthier-Schaad, Y.; Sechler, A.; Knorr, D. (1999) Detection of Clavibacter michiganensis subsp. sepedonicus in potato tubers by BIO-PCR and an automated real-time fluorescence detection system.
Plant Disease, 83(12), 1095-1100.