Dig-labelled PCR to detect Clavibacter michiganensis subsp. sepedonicus
A digoxigenin-labelled PCR method has been developed to detect Clavibacter michiganensis subsp. sepedonicus (EPPO A2 quarantine pest) in seed potato tubers and stem tissues. Compared with nested PCR and ELISA, it was found that it has a detection sensitivity close to that of nested PCR (and higher than ELISA), and that it was as easy to use as ELISA. This method can be used to detect symptomless infection in field potato tubers.
Lee, I.M.; Lukaesko, L.A.; Maroon, C.J.M. (2001) Comparison of dig-labeled PCR, nested PCR, and ELISA for the detection of Clavibacter michiganensis subsp. sepedonicus in field-grown potatoes.
Plant Disease, 85(3), 261-266.