PCR method to identify Meloidogyne chitwoodi, M. fallax and M. hapla
A PCR method for Meloidogyne chitwoodi (EPPO A2 list), M. fallax (EPPO A2 list) and M. hapla was developed as part as an EU project to develop management tools for the two which are quarantine nematodes in Europe. A set of species-specific primers was developed for the differentiation of the three species by one-step multiplex PCR. Amplifications were achieved on bulk samples of DNA and on individual nematodes (juveniles, males or females). Unlike other techniques, which require the use of several methods to differentiate between the three species, this method worked alone, at low levels of contamination. It can be used to detect the three species in root samples and in dot-blotted DNA.
Wishart, J.; Phillips, M.S.; Blok, V.C. (2002) Ribosomal intergenic spacer: a polymerase chain reaction diagnostic for Meloidogyne chitwoodi, M. fallax and M. hapla.
Phytopathology, 92(8), p 884-892.