Real-time quantitative PCR for Xylella fastidiosa on citrus
In Brazil, a real-time quantitative PCR method was developed for Xylella fastidiosa (EPPO A1 list). It was used on artificially infested Citrus sinensis and Citrus reticulata (by inoculation or grafting), using known resistant and susceptible cultivars. This method allowed quantification of X. fastidiosa, and determination of its temporal and spatial distribution, in citrus tissues. The method is straightforward, sensitive and reproducible. It also confirmed earlier results obtained on grapevine according to which multiplication and spread of X. fastidiosa is faster within more susceptible cultivars. The authors believe that this method is convenient for the study of different aspects of the development of X. fastidiosa in citrus and could also be used in citrus breeding programme to identify sources of resistance or tolerance to X. fastidiosa.
Oliveira, C; Vallim, M.A.; Semighini, P.; Araújo, W.L.; Goldman, G.H.; Machado, M.A. (2002) Quantification of Xylella fastidiosa from citrus trees by real-time polymerase chain reaction assay.
Phytopathology, 92(10), p 1048-1054