PCR method to differentiate between Tomato yellow leaf curl Sardinia and Tomato yellow leaf curl begomoviruses
Tomato yellow leaf curl-Sardinia and Tomato yellow leaf curl-Israel begomoviruses (TYLCV-Sar and TYLCV, both EPPO A2 quarantine pests) cannot be differentiated on the basis of their symptomatology, although TYLCV-Is is more aggressive and causes greater economic losses. A rapid PCR method has been developed in Spain to differentiate between the two virus species. A combination of primers (specific to any isolate of TYLCV and of TYLCV-Sar) was selected to develop a duplex PCR method which can detect either TYLCV-Sar or TYLCV.
Martínez-Culebras, P.V.; Font, I.; Jordá, C. (2001) A rapid PCR method to discriminate between Tomato yellow leaf curl virus isolates.
Annals of applied Biology, 139(2), 251-257.