Further studies on an Italian isolate of Pepino mosaic potexvirus
Studies were done in Italy on the experimental host range, possible seed transmission and detection of Pepino mosaic potexvirus (EPPO Alert List). The experimental host range of an Italian isolate of the virus (from Lycopersicon esculentum cv. Camona, Sardinia) was tested. Systemic infection was obtained only on Solanaceae with severe symptoms on Nicotiana megalosiphon and Datura stramonium, and mild mosaic in N. benthamiana, Solanum melongena (aubergine) and tomato. It is noted that in its experimental host range, the Italian isolate differed markedly from the Peruvian isolate from pepino (S. muricatum) and to a lesser extent from a Spanish isolate (also collected from tomato). In particular, capsicum (Capsicum annuum) and potato (S. tuberosum) were not found susceptible to this Italian isolate. It is stressed that further studies are needed to assess the biodiversity of Pepino mosaic potexvirus isolates from tomato in Europe. Seed transmission experiments confirmed that Pepino mosaic potexvirus is not seed-transmitted. Detection methods were also studied and it was observed that the virus was efficiently detected in tomato (and other hosts) by different ELISA procedures and by a rapid field immunochromatographic system based on lateral flow.
Salomone, A.; Roggero, P. (2002) Host range, seed transmission and detection by ELISA and lateral flow of an Italian isolate of Pepino mosaic potexvirus.
Journal of Plant Pathology, 84(1), 65-68.