EPPO Global Database

EPPO Reporting Service no. 05 - 2002 Num. article: 2002/085

Diagnostic procedure for Clavibacter michiganensis subsp. sepedonicus

A new PCR assay has been developed in Spain to detect Clavibacter michiganensis subsp. sepedonicus (EPPO A2 quarantine pest). This new procedure (called TP-RADP) uses two primers to amply the 16S rDNA gene. The PCR product obtained then produces a band pattern in electrophoresis which is specific of C. m. subsp. sepedonicus and identical for all strains.


Rivas, R.; Velázquez, E.; Palomo, J.L.; Mateos, P.F.; García-Benavides, P.; Martínez-Molina, E. (2002) Rapid identification of Clavibacter michiganensis subspecies sepedonicus using two primers random amplified polymorphic DNA (TP-RAPD) finger prints.
European Journal of Plant Pathology, 108(2), 179-184.