Diagnostic procedure for Clavibacter michiganensis subsp. sepedonicus
A new PCR assay has been developed in Spain to detect Clavibacter michiganensis subsp. sepedonicus (EPPO A2 quarantine pest). This new procedure (called TP-RADP) uses two primers to amply the 16S rDNA gene. The PCR product obtained then produces a band pattern in electrophoresis which is specific of C. m. subsp. sepedonicus and identical for all strains.
Sources
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Rivas, R.; Velázquez, E.; Palomo, J.L.; Mateos, P.F.; García-Benavides, P.; Martínez-Molina, E. (2002) Rapid identification of Clavibacter michiganensis subspecies sepedonicus using two primers random amplified polymorphic DNA (TP-RAPD) finger prints.
European Journal of Plant Pathology, 108(2), 179-184.
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