Detection of Pepino mosaic potexvirus
In Spain, an RT-PCR assay combined with RFLP was developed to detect specifically Pepino mosaic potexvirus (EPPO Alert List) and differentiate between isolates. This technique was used successfully in tomato samples and in weeds (Amaranthus, Malva parviflora, Nicotiana glauca, Solanum nigrum, Sonchus oleraceus). This confirmed the presence of the virus in these weed species. In RFLP studies, 3 different types of isolates were obtained. The authors concluded that RT-PCR combined with RFLP is a useful tool which will help to understand the epidemiology and distribution of PepMV, as well as the variability of its isolates.
Martínez-Culebras, P.V.; Lázaro, A.; Abad Campos, P.; Jordá, C. (2002) A RT-PCR assay combined with RFLP analysis for detection and differentiation of isolates of Pepino mosaic virus (PepMV) from tomato.
European Journal of Plant Pathology, 108, 887-892.