EPPO Global Database

EPPO Reporting Service no. 04 - 2003 Num. article: 2003/065

Detection methods for Ralstonia solanacearum

Ralstonia solanacearum (EPPO A2 quarantine pest) is an heterogeneous species. Comparison of ITS sequences have shown that strains could be separated into 2 divisions, in accordance with RFLP data and 16S rRNA gene sequences: division I and II (including strains of biovar 2 race 3). Specific primers were selected for the identification of these two divisions. In addition, a new multiplex PCR was developed for the routine detection of R. solanacearum in host plants (in particular in potato tubers).


Pastrik, K.H.; Elphinstone, J.G.; Pukall, R. (2002) Sequence analysis and detection of Ralstonia solanacearum by multiplex PCR amplification of 16S-23S ribosomal intergenic spacer region with internal positive control.
European Journal of Plant Pathology, 108(9), 831-842.