First report of Globodera rostochiensis in Bosnia and Herzegovina
In Bosnia and Herzegovina, surveys for potato cyst nematodes (Globodera rostochiensis and G. pallida – both EPPO A2 List) were carried out in 2008, 2009 and 2010. Soil samples consisting of 100 cores of 4 to 5 mL of soil (approximately 500 g of soil in total) were taken following a grid pattern throughout the potato plots. Samples were processed in the laboratory and nematode species identification was based on morphological characters and real-time PCR. In 2008, 5 locations were sampled and no Globodera species were found. In June 2009, 17 soil samples were collected from 7 locations and 2 viable cysts of G. rostochiensis were found in 1 sample from Tihaljina (Grude municipality). In May and June 2010, 110 soil samples were collected from 90 locations and 5 viable cysts of G. rostochiensis were detected in 1 sample from the municipality of Čapljina. It is concluded that for the moment G. rostochiensis is not widely spread, suggesting that the infestation is relatively recent. It is also noted that measures should be taken to maintain its populations at low levels and prevent its further spread. G. pallida was not found.
The situation of Globodera rostochiensis in Bosnia and Herzegovina can be described as follows: Present, first found in 2009, detected in a small number of soil samples.
Ostojić I, Grubišić D, Zovko M, Miličević T, Gotlin T (2011) First report of the golden potato cyst nematode, Globodera rostochiensis in Bosnia and Herzegovina. Plant Disease 95(7), p 883.