Molecular techniques to distinguish Liriomyza trifolii from L. sativae
In Japan, Liriomyza trifolii and L. sativae (Diptera: Agromyzidae – both EPPO A2 list) were first found in the early 1990s and in 1999, respectively. They are now believed to be widespread and dominant on some vegetable crops, and they can occur at the same locations. A multiplex PCR method, amplifying a region of the mitochondrial cytochrome oxidase gene has been developed in Japan to differentiate L. trifolii rapidly from L. sativae.
Sources
Miura, K.; Tagami, Y.; Ohtaishi, M.; Iwasaki, A. (2004) Application of molecular techniques to distinguish Liriomyza trifolii from L. sativae (Diptera: Agromyzidae) on tomato cultivation in Japan.
Journal of Economic Entomology, 97(3), 964-969.