Situation of several quarantine pests in Croatia in 2003
The NPPO of Croatia recently informed the EPPO Secretariat of the situation of the following quarantine pests in 2003:
Clavibacter michiganensis subsp. sepedonicus and Ralstonia solanacearum (A1 quarantine list in Croatia, EPPO A2 list)
During the 2003 growing-season, surveys were carried out for these two potato bacteria. They were performed in 13 Croatian counties and covered 990 ha of potato fields. Visual inspections were done in potato crops, including also seed potato production and potato stores. In addition, tuber samples were collected for testing. From May to December 2003, samples (each of 200 tubers) were collected and tested by IF. 172 IF tests were performed (86 for C. michiganensis subsp. sepedonicus and 86 for R. solanacearum) and all gave negative results.
The status of Clavibacter michiganensis subsp. sepedonicus and Ralstonia solanacearum in Croatia is declared as follows: Absent, confirmed by survey.
Diabrotica virgifera (A2 quarantine list in Croatia, EPPO A2 list)
The 2003 situation of this pest has already been presented in EPPO RS 2004/058.
The status of Diabrotica virgifera in Croatia is declared as follows: Present, in the eastern region of Croatia, spreading towards the west.
Globodera rostochiensis (A2 quarantine list in Croatia, EPPO A2 list)
During the 2003 growing-season, visual inspections were carried out in potato fields and soil samples were collected in 17 Croatian counties (204 locations). 3200 soil samples were analysed and Globodera rostochiensis was found in 33 samples originating from 4 counties: Zagrebačka (Pećno, 8 infested samples), Varaždinska (Nova Ves, 9 samples), Primorsko-goranska (Ravna Gora, 4 samples) and Međimurska (Gardinovec and Pribislavec, 12 samples). As the pest was found for the first time in Nova Ves and Ravna Gora, both morphological characters and molecular testing were used to identify cysts in these newly infested areas. Pathotypes were identified by using bioassays. Ro1, Ro2 and Ro3 were found (Ro2 and Ro3 were found only at Ivanovec).
The status of Globodera rostochiensis in Croatia is declared as follows: Present in limited areas, under surveillance.
Globodera pallida (A1 quarantine list in Croatia, EPPO A2 list)
Globodera pallida was found for the first time in Croatia during the 2003 growing season. Systematic surveys on potato cyst nematodes were performed and included: visual inspections of potato fields, collection of soil samples (from 17 Croatian counties - 204 locations) and analysis of 3200 samples. 33 soil samples were found infested by potato cyst nematodes. Cysts from 3 counties (Međimurska županija, Varaždinska županija, Primorsko-goranska županija) were analysed using bioassays to determine pathotypes present. These tests confirmed the presence of G. pallida pathotypes Pa2 and Pa3. The first determination of G. pallida was confirmed in soil samples collected from 3 locations, all situated in the northwestern part of Croatia: Sivice and Ivanovec (county Međimurska županija) and Vidovec (Varaždinska županija).
The status of Globodera pallida in Croatia is declared as follows: Present, first found in 2003, only in the northwest region.
Liriomyza huidobrensis (A2 quarantine list in Croatia, EPPO A2 list) and L. sativae (A1 quarantine list in Croatia, EPPO A2 list)
From April to December 2003, surveys on quarantine pests of glasshouses were carried out in 18 counties. Visual inspections of plants growing under glasshouse (Lycopersicon esculentum, Capsicum annuum, Cucumis sativus, Chrysanthemum, Dianthus and Dahlia) were performed at 59 locations (72 glasshouses). In addition, 150 yellow sticky traps were placed in 28 glasshouses. Liriomyza huidobrensis was found for the first time in Croatia in 2002*1, at 3 locations in Dalmatia (Duilovo, Turanj and Trogir). In 2003, the pest was found at the same locations and for the first time in 3 new other places: Dubrovnik (county Dubrovačko-neretvanska županija), Lučko (Zagrebačka županija), and Knežine (Splitsko-dalmatinska županija). It is assumed that the pest was spread with planting material. L. sativae was not found.
The status of Liriomyza huidobrensis in Croatia is declared as follows: Present in a few glasshouses.
The status of Liriomyza sativae in Croatia is declared as follows: Absent, confirmed by survey.
Synchytrium endobioticum (A1 quarantine list in Croatia, EPPO A2 list)
Samples of potato tubers were collected during the 2003 growing-season. These samples were tested (206 mycological tests were performed) for the presence of Synchytrium endobioticum. The fungus was not detected.
The status of Synchytrium endobioticum in Croatia is declared as follows: Absent, confirmed by survey.
Thrips palmi (A1 quarantine pest in Croatia, EPPO A1 list)
From April to December 2003, surveys on quarantine pests of glasshouses were carried out in 18 counties. Visual inspections of plants growing under glasshouse (Capsicum annuum, Cucumis sativus, Chrysanthemum, Cyclamen, Ficus and Orchidaceae) were performed at 59 locations (72 glasshouses). In addition, 33 blue sticky traps were placed in 8 glasshouses. During visual inspection, 34 samples of adult thrips were collected and determined under the microscope. Thrips palmi was not found.
The status of Thrips palmi in Croatia is declared as follows: Absent, confirmed by survey.
* New record according to the EPPO Secretariat.
Sources
NPPO of Croatia, 2004-06.