Tissue blot immunoassay to detect Tomato spotted wilt tospovirus
A tissue blot immunoassay (TBIA) was developed in USA to detect Tomato spotted wilt tospovirus (TSWV - EPPO A2 list) in Ranunculus asiaticus tubers (as the virus can persists in tubers after harvest) and in plant tissues of other ornamentals. In terms of accuracy and reliability, TBIA gave similar results to DAS-ELISA. With this method, TSWV was successfully detected in: Begonia x hiemalis, Chrysanthemum, Eustoma, Impatiens, Malva parviflora, Senecio cruentus, Datura stramonium and Tropaeolum. However, false positive results were obtained with Kalanchoe and Limonium latifolium. It was concluded that TBIA was a useful tool in TSWV management programmes to identify infected plants.
Sources
Whitfield, A.E.; Campbell, L.R.; Sherwood, J.L.; Ullman, D.E. (2003) Tissue blot immunoassay for detection of Tomato spotted wilt tospovirus in Ranunculus asiaticus and other ornamentals.
Plant Disease, 87(6), 618-622.