New detection method for Strawberry vein banding caulimovirus
In certification schemes for strawberry, routine detection of Strawberry vein banding caulimovirus (EPPO A2 list) was, until now, essentially based on biological testing on Fragaria indicator clones. Molecular techniques have been developed but, so far, it was recommended to use them in combination (PCR together with molecular hybridization to a SVBV-specific probe). Recently, a real-time NASBA method (nucleic acid sequence base amplification) was developed to detect SVBV specifically in strawberry plants, and compared with the other existing tests. The authors felt that this method was rapid, sensitive, and reliable. Although it was tested on a limited number of SVBV isolates, this method offers good prospects for routine detection of the virus in strawberry planting material.
Vašková, D.; Špak, J.; Klerks, M.M.; Schoen, C.D.; Thompson, J.R.; Jelkmann, W. (2004) Real-time NASBA for detection of Strawberry vein banding virus. European Journal of Plant Pathology, 110(2), 213-221.