Serological tests for the detection of Cucurbit yellow stunting disorder crinivirus
In order to detect Cucurbit yellow stunting disorder crinivirus (CYSDV - EPPO Alert List), polyclonal antibodies have been prepared in Lebanon. Recombinant DNA technology was used to express CYSDV coat protein in bacterial cultures, to purify it and to use it as an antigen. The antiserum produced was used in different serological tests: tissue blot immunoassay (TBIA), dot blot immunoassay (DBIA) and in two different ELISA techniques. The antiserum gave good results with all tested methods, and could detect CYSDV in infected cucumber and melon plant tissues. However, the authors concluded that TBIA could be recommended for large scale surveys, as it was found very specific, easier to use and more economical.
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Note: In this paper, CYSDV is reported as present in Syria. The EPPO Secretariat had previously no data on the presence of this pathogen.
Sources
Hourani, H.; Abou-Jawdah, Y. (2003) Immunodiagnosis of Cucurbit yellow stunting disorder virus using polyclonal antibodies developed against recombinant coat protein.
Journal of Plant Pathology, 85(3), 197-204.