Detection methods for Cucumber vein yellowing ipomovirus
Two detection methods for Cucumber vein yellowing ipomovirus (CVYV - EPPO A2 list) have been developed in Spain. The first detection method used an RNA-specific probe for hybridization with nucleic acids extracted from infected plants. The second method used polyclonal antisera raised against recombinant viral coat protein expressed in bacteria. These specific antibodies were able to detect CVYV particles in plant extracts. For both methods, no cross-reactions were observed with other cucurbit-infecting viruses (Cucumber mosaic cucumovirus, Cucurbit yellow stunting disorder crinivirus, Papaya ringspot potyvirus, Watermelon mosaic potyvirus, Zucchini yellow mosaic potyvirus) and their sensitivities were considered sufficient for routine diagnosis of CVYV in plants.
Sources
Martínez-García B, Marco CF, Goytia E, López-Abella D, Serra MT, Aranda MA, López-Moya JJ (2004) Development and use of detection methods specific for Cucumber vein yellowing virus (CVYV).
European Journal of Plant Pathology, 110(8), 811-821.