PCR detection of latent infections of Glomerella acutata on strawberry
Glomerella acutata (anamorph Colletotrichum acutatum – EU annexes) can easily be spread with visibly or latently infected strawberry planting material (e.g. cold-stored transplants) moving in trade. A PCR method using specific primers has been developed in Finland to detect G. acutata. This method allows detection of G. acutata in symptomatic and asymptomatic plant parts as well as in artificially or naturally infected strawberry tissues. The pathogen could be detected on ripe/unripe berries, runners, petioles and parts of crowns. It was felt that this method could usefully be used for phytosanitary inspections.
Sources
Parikka P, Lemmetty A (2004) Tracing latent infection of Colletotrichum acutatum on strawberry by PCR.
European Journal of Plant Pathology, 110(4), 393-398.
|