New real-time PCR test to detect Guignardia citricarpa
A new real-time PCR test was developed in the Netherlands to specifically detect Guignardia citricarpa (EPPO A1 List) on citrus fruits. When compared with conventional PCR, the new protocol was found more sensitive, less laborious and faster. It could also be adapted to portable thermocyclers to be used for on-site detection. It is considered that this new real-time PCR protocol efficiently detects G. citricarpa in lesions from suspect fruit samples and could be used during routine import inspections.
van Gent-Pelzer MPE, van Brouwershaven IR, Kox LFF, Bonants PJM (2007) TaqMan PCR method for routine diagnosis of the quarantine fungus Guignardia citricarpa on citrus fruits. Journal of Phytopathology 155(6), 357-363.