EPPO Global Database

EPPO Reporting Service no. 11 - 2007 Num. article: 2007/222

New real-time PCR test to detect ‘Candidatus Phytoplasma mali’

A new real-time PCR test has been developed to detect specifically ‘Candidatus Phytoplasma mali’ (phytoplasma associated with apple proliferation – EPPO A2 List). Comparisons were made with other real-time PCR protocols and conventional PCR (using fU5/rU3 primers). The new real-time PCR test was found highly sensitive (10 to 100 fold more sensitive than conventional PCR) and its specificity was improved compared to other real-time PCR tests. The authors concluded that the new method had a good potential for routine testing, in particular in certification schemes.


Aldaghi M, Massart S, Roussel S, Jijakli MH (2007) Development of a new probe for specific and sensitive detection of ‘Candidatus Phytoplasma mali’ in inoculated apple trees. Annals of Applied Biology 151(2), 251-258.