Real-time multiplex detection of Meloidogyne chitwoodi and M. fallax
A multiplex real-time PCR assay has been developed in the Netherlands for the simultaneous detection of Meloidogyne chitwoodi and M. fallax (both EPPO A2 List). This PCR method could detect both species in DNA samples extracted from batches of juvenile nematodes, from single juveniles and from infected plant material (potato tubers and crocus bulbs). Although the preliminary results were found satisfactory, further research is needed to verify that this real-time PCR method can reliably be used on field samples.
Zijlstra C, van Hoof RA (2006) A multiplex real-time Polymerase Chain Reaction (TaqMan) assay for the simultaneous detection of Meloidogyne chitwoodi and M. fallax. Phytopathology 96(11), 1255-1262.