A molecular diagnostic key to identify seven species of Meloidogyne
A molecular diagnostic key was developed in the United Kingdom to distinguish between 7 of the most common and economically important Meloidogyne species: Meloidogyne incognita, M. javanica, M. arenaria, M. mayaguensis, M. hapla, M. chitwoodi (EPPO A2 List) and M. fallax (EPPO A2 List). This key is composed of three steps of PCR tests using different combinations of primers that were previously validated and shown to work reliably and specifically. It can be used with bulk extraction of DNA as well as DNA obtained from single juvenile or adult individuals. The authors considered that this molecular diagnostic key could be further expanded to include other Meloidogyne species.
Adam MAM, Phillips MS, Blok VC (2007) Molecular diagnostic key for identification of single juveniles of seven common and economically important species of root-knot nematode (Meloidogyne spp.). Plant Pathology 56(1), 190-197.